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. 2012 Jan 10;6(1):e1466. doi: 10.1371/journal.pntd.0001466

Figure 3. Multiple intracellular O. tsutsugamushi within antigen presenting cells (APCs) in the superficial dermis.

Figure 3

APCs are characterised by MHC class II receptor (HLADR) positivity and were associated with intracellular O. tsutsugamushi in admission samples of an eschar from a patient with acute scrub typhus. Panel A: HLADR-positive cells in red, O. tsutsugamushi in green. Panels B and C: Laser Scanning Micrographs. Panel B depicts the same infected cell as in Panel A as a 0.3 µm thin section. Panel C shows a 3D stack projection (of 0.3 µm thin sections) of intracytosolic O. tsutsugamushi (in green), with small, high-density granules with high refractive index, staining positively for O. tsutsugamushi antigen. Patient TM2193, magnification ×400, LSM inserts ×1000, double-immunolabeling: HLADR in red, O. tsutsugamushi in green and DAPI nuclear counterstain in blue.