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. 2011 Oct 21;63(2):669–679. doi: 10.1093/jxb/err255

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© 2011 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 1. — Characterization of the suc2-5 mutant. (A) Scheme of the SUC2 gene with the confirmed insertion sites of suc2-4 (SALK_038124) and suc2-5 (SALK_087046). Black, exons; white, introns; LB, left border; small black arrows, primer binding sites and primer orientation. (B) Semi-quantitative RT-PCRs on total RNA from wt and suc2-5 plants showing the abundance of a SUC2 mRNA fragment spanning the insertion site (SUC2a, primers #1 and #2) and of an mRNA fragment upstream from the insertion site (SUC2b, primers #1 and #3). ACTIN2 transcript (ACT2) was used as control for amounts of cDNA. (C, D) Phenotype of wt, heterozygous (he) and homozygous (ho) suc2-5 plants at 27 d after germination (dag) (C) and at 41 dag (D). Arrow indicates the tiny homozygous plant. (E) Homozygous suc2-5 plant (48 dag) with anthocyanin accumulation at the leaf margins. Edge length of pots: 6.5 cm.