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. 2011 Oct 21;63(2):669–679. doi: 10.1093/jxb/err255

Fig. 5.

Fig. 5.

Srt1-RFP localizes to the plasma membrane of Arabidopsis mesophyll protoplasts where it catalyses the uptake of radiolabelled sucrose. (A–C) Optical sections of an Arabidopsis protoplast cotransformed with an Srt1-RFP and an GFP-INT4 construct. (A) Localization of Srt1-RFP. (B) Localization of GFP-INT4. (C) Detection of chloroplasts by chlorophyll autofluorescence. (D) Merge of (A–C). Bar=10 μm. (E) Uptake of 14C-sucrose into Arabidopsis protoplasts expressing GFP-INT4 (control) or Srt1-RFP (Srt1). Srt1 and control data show results from two different protoplast transformations and two transport tests per transformation, respectively.