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Basal levels of HSP mRNAs in the cochlea of Hsf1−/− vs. Hsf1+/− mice. qRT-PCR was performed on total RNA from the cochleae of unstressed Hsf1−/− (n = 9) and Hsf1+/− mice (n = 7), from six independent experiments. Ratios indicate the HSP mRNA levels in Hsf1−/− mice compared to their paired Hsf1+/− mice within each experiment. Ratios were subjected to paired Student’s t test. Asterisks denote statistically significant differences: ***p < 0.001. Bars indicate SEM.
