Fig. 9.
Western blot analysis show decreased expression of Chk1 and Chk2. (A) Digitoxin and D6-MA down-regulated Chk1 and Chk2 in a dose dependent manner. (B) D6-MA is more potent than digitoxin in inhibiting Chk1 expression (F=10.17, p<0.001). Quantification of Chk1 blots shows decreased expression following treatment (*p<0.05 compared 0 nM, †p<0.05 compared to respective digitoxin treatment). (C) D6-MA is more potent than digitoxin in inhibiting Chk2 expression (F=7.982, p<0.001). Quantification of Chk2 blots shows decreased expression following treatment (*p<0.05 compared 0 nM, †p<0.05 compared to respective digitoxin treatment).