Fig. 4.

Whole mount observations of AM1-43 microinjected tongues. (A-D) Twenty-four hour after the in utero injections of AM1-43, positive localizations are detected in the circumvallate papilla (CVP) and fungiform papillae regions. (E-H) After intra-peritoneal injections of AM1-43 for 24 h, fungiform papillae and CVP show much stronger positive reactions of AM1-43 than those of embryonic day 16 (E16). PN3, postnatal day 3; BF, bight field; FL, fluorescent. Scale bars=1 mm (A, B, E, F), 250 µm (C, D), 500 µm (G, H).