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. Author manuscript; available in PMC: 2012 Nov 9.
Published in final edited form as: Structure. 2011 Nov 9;19(11):1582–1590. doi: 10.1016/j.str.2011.10.003

Figure 3.

Figure 3

Six structures derived from the data set of 586,329 cryo-EM projection images of the Thermus Thermophilus 70S•tRNA•EF-Tu•GDP•kirromycin complex obtained with K-means clustering using factorial coordinates derived from the first three dominating eigenvolumes and 3D reconstruction according to 2D projection assignments to clusters (a-f). Structures (a,c-f) of the ribosome are in classical conformation, while sub-population (b) is in the rotated conformation (RTS). (g) - RTS between structure (b) shown in light brown and structure (c) shown in gold. CP, central protuberance; SB, stalk base; L1, protein L1. Blue – 50S subunit, transparent yellow – 30S subunit, brown – E-site tRNA, green – P-site tRNA, red – EFG. See also Figures S2-S7 and Tables S1 and S2.