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PCR conditions for aldose reductase and β-actin genes. Previously published primer sequences were verified by NCBI BLAST. Amplification was conducted for 26 cycles.
| Gene | Sequence of primers (5′ to 3′) | Amplicon (bp) | Annealing temp. (°C) |
|---|---|---|---|
| Aldose reductase | Sense: CCCAGGTGTACCAGAATGAGA | 580 | 53.1 |
| Antisense: TGGCTGCAATTGCTTTGATCC | |||
|
| |||
| β-actin | Sense: CCGTAAAGACCTCTATGCCA | 299 | 50.8 |
| Antisense: AAGAAAGGGTGTAAAACGCA | |||
