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. 2011 Jul 22;1(7):e29. doi: 10.1038/bcj.2011.29

Figure 2.

Figure 2

Inhibition of JAK2-mediated signaling and induction of apoptosis in Ba/F3-JAK2V617F cells by NS-018. (a) Ba/F3-JAK2V617F cells were treated with increasing concentrations of NS-018 for 3 h and then lysed. Lysate was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to a Polyvinylidene fluoride membrane and probed with the indicated antibodies. (b) Ba/F3-JAK2V617F cells were treated with 0–300 n NS-018 for 48 h and tested for apoptosis by staining with annexin V/propidium iodide (PI). (c) Ba/F3-JAK2V617F cells were treated with NS-018 for 29 h before DNA extraction and DNA fragmentation was assessed by agarose gel electrophoresis. M indicates the DNA size marker.