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Effect of NS-018 on erythroid colony formation by PV patient cells. Mononuclear cells isolated from the peripheral blood of PV patients with the JAK2V617F mutation (n=4) or healthy volunteers (n=3) were treated with increasing concentrations of NS-018 in methylcellulose-based media with erythropoietin (a) or without erythropoietin (b). BFU-Es were counted on day 14. Values represent the mean±s.e.m. Statistical significance was assessed by the t-test (*P<0.05).
