Figure 4.
Effect of NS-018 on survival and splenomegaly in a mouse Ba/F3-JAK2V617F disease model. Disease was established in BALB/c nude mice by intravenous injection of 1 × 106 Ba/F3-JAK2V617F cells. (a) Survival curves. NS-018 was administered by oral gavage twice a day for 11 days at the indicated doses. Statistical significance was assessed by the log-rank test (*P<0.01 vs vehicle, eight mice per group). (b) Mice inoculated with Ba/F3-JAK2V617F were orally administered with the indicated doses of NS-018 twice a day. Uninoculated control mice (sham) received vehicle only. The day after the completion of 8 days of administration, spleens were removed and weighed. Values represent the mean±s.e.m. Statistical significance was assessed by the t-test (#P<0.01 vs sham) and Dunnett's test (*P<0.01 vs vehicle, five mice per group).