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. 2011 Jul 22;1(7):e29. doi: 10.1038/bcj.2011.29

Figure 5.

Figure 5

Figure 5

Effect of NS-018 in JAK2V617F transgenic mice. JAK2V617F transgenic mice (TG) were divided into three groups and orally administered with vehicle, 25 mg/kg NS-018, or 50 mg/kg NS-018 (n=34, 37, and 36, respectively) twice a day for 24 weeks. Wild-type mice (WT) received vehicle only (n=27). (A) White blood cell (WBC), (C) red blood cell (RBC) and (D) platelet (PLT) counts were monitored monthly. Values represent the mean±s.e.m. Statistical significance was assessed by the t-test (*P<0.05, **P<0.01 vs TG vehicle). (B) Differences in cellular composition. Nucleated cells from peripheral blood were stained with fluorescently conjugated antibodies specific for Mac1/Gr1 (myeloid cells), B220 (B cells) and CD3 (T-cells) and analyzed by flow cytometry. (E) The weight of liver and spleen were measured at the end of the treatment period. Values represent the mean±s.e.m. Statistical significance was assessed by the t-test (#P<0.01 vs WT vehicle) or Dunnett's test (*P<0.05, **P<0.01 vs TG vehicle). (F) Spleen cells were stained with fluorescently conjugated antibodies specific for Mac1/Gr1 (myeloid cells), B220 (B cells) and CD3 (T-cells) and analyzed by flow cytometry. (G) After NS-018 treatment, tissue sections were prepared from spleen (a–d; original magnification × 40), liver (e–h; magnification × 200) and lung (i–l; magnification × 200) for staining with hematoxylin and eosin. (H) The body weight of the mice was monitored weekly. (I) The total cholesterol in the serum at the end of the study. Values represent the mean±s.e.m. Statistical significance was assessed by the t-test (#P<0.01 vs WT vehicle) or Dunnett's test (*P<0.01 vs TG vehicle). (J) Survival curves of mice. Statistical significance was assessed by the log-rank test (#P<0.01 vs WT vehicle, *P<0.01 vs TG vehicle).