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. 2011 Jun 24;1(6):e26. doi: 10.1038/bcj.2011.24

Figure 2.

Figure 2

MiR-146a and CXCR4 expression in purified CD34+ HPCs induced to monocytic (Mo) differentiation. (a, b) CD34+ HPCs (day 0) proliferation and differentiation were controlled, respectively, by growth curve analysis (a) and expression of CD11b (b). (c) Real time PCR analysis of CXCR4 mRNA expression in HPCs grown in liquid-phase unilineage Mo cultures. (d) FACS of CXCR4 membrane protein expression in HPCs during unilineage Mo cultures. The geometric mean fluorescence intensity is indicated. (e) Real time PCR analysis of miR-146a expression in HPCs during Mo differentiation. (a, b) One representative experiment out of three is shown. (c–e) The results of three independent experiments (mean±s.e.m. values) are shown.