Figure 1.

The proximal tibial growth plate is thinned in dasatinib-treated animals. Nine-month-old female Sprague–Dawley rats were treated for 12 weeks with dasatinib (5 mg/kg per day) or vehicle (10% DMSO/90% PEG 300). Six animals per group were humanely killed after 4, 8 and 12 weeks of treatment and right tibiae were collected for histological analysis. Samples were embedded in paraffin and 5-μm sections were stained with safranin O/fast green. Growth plate thickness was measured at five equidistant points across the centre of the growth plate. Box plots depict median, 25th and 75th percentiles±range (n=4–6). ^*P<0.05 (Student's t-tests).