Figure 3.

CTL clones stimulated with HLA-A2⁺ ITD⁺ DC_AML recognize leukemia cell lines and blasts. CD8⁺ T cells from a healthy HLA-A2⁻ donor were primed with allogeneic HLA-A2⁺ DC_AML and specific T cells were cloned. (a) In an IFN-γ ELISpot, the three CTL_AML clones were co-incubated with K562tA2, K562 and HLA-A2⁺ BV173 cells, and the resulting spots were counted. (b) An IFN-γ ELISpot was performed using allogeneic HLA-A2⁻ PBMC and blasts, as well as HLA-A2⁺ blasts as targets and the three CTL_AML clones as effector cells.