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. 2011 Mar 18;1(3):e11. doi: 10.1038/bcj.2011.12

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Copyright © 2011 Macmillan Publishers Limited

This work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/

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Reactivity of CTL clones directed against PR-3, RHAMM and Survivin, as well as CTL_AML clones towards DCs nucleofected with FLT3-WT plus and minus FLT3-siRNA or FLT3-ITD. All approaches were carried out with and without the addition of FL. (a, b) HLA-A2⁺ DCs from a healthy donor were nucleofected with FLT3-WT-mRNA or FLT3-ITD-mRNA and co-cultivated with the different CTL clones with and without the addition of FL. Furthermore, the FLT3-WT⁺ DCs were additionally transfected with FLT3-siRNA. The supernatants were tested in an IFN-γ ELISA. (a, b) show the results of the ELISA with the CTL clones directed against PR-3, RHAMM and Survivin, as well as with the CTL_AML clone nos 3, 8 and 12.