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. 2011 Dec;6(12):2766–2774. doi: 10.2215/CJN.06820711

Figure 5.

Figure 5.

Western blot analyses showing maturation of wild-type and mutant uromodulin over time. (A) Detection of uromodulin in total cell lysates from HEK293 cells transfected with WT, indel, or C150S UMOD, 4 to 72 hours post-transfection. The lower panels show β-actin loading controls. (B) Detection of uromodulin in the same media at 12, 24, 48, and 72 hours. Less mature (m) uromodulin can be detected in the indel transfected than in WT media at 24, 48, and 72 hours. The immature 82-kD precursor (p) is prominent in the C150S transfected cells. ns, nonspecific band. Both panels are from the same gel.