Skip to main content
. Author manuscript; available in PMC: 2012 Nov 16.
Published in final edited form as: J Am Chem Soc. 2011 Oct 21;133(45):18447–18451. doi: 10.1021/ja208025e

Table 2.

Bacterial strain genotypes tested for ability to replicate xDNA segments.

Strain Deleted Gene Function/Gene Product Genotype
BW25113 -- F-, Δ(araD-araB)567, ΔlacZ4787(∷rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514
JW2703-2 mutS methyl-mediated mismatch repair F-, Δ(araD-araB)567, ΔlacZ4787(∷rrnB-3), λ-, ΔmutS738∷kan, rph-1, Δ(rhaD-rhaB)568, hsdR514
JW0704-1 nei endonuclease VII F-, Δ(araD-araB)567, ΔlacZ4787(∷rrnB-3), Δnei-764∷kan, λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514
JW2928-1 mutY adenine glycosylase: G-A repair F-, Δ(araD-araB)567, ΔlacZ4787(∷rrnB-3), λ-, ΔmutY736∷kan, rph-1, Δ(rhaD-rhaB)568, hsdR514
JW3610-2 mutM (fpg) formamidopyrimidine-DNA glycosylase F-, Δ(araD-araB)567, ΔlacZ4787(∷rrnB-3), λ-, ΔmutM744∷kan, rph-1, Δ(rhaD-rhaB)568, hsdR514
JW4019-2 uvrA excision nuclease: molecular matchmaker F-, Δ(araD-araB)567, ΔlacZ4787(∷rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, ΔuvrA753∷kan, hsdR514
JW0221-1 dinB Pol IV F-, Δ(araD-araB)567, ΔdinB749∷kan, ΔlacZ4787(∷rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514
JW1173-1 umuC Pol V F-, Δ(araD-araB)567, ΔlacZ4787(∷rrnB-3), λ-, ΔumuC773∷kan, rph-1, Δ(rhaD-rhaB)568, hsdR514
JW0059-1 polB (dinA) Pol II F-, ΔpolB770∷kan, Δ(araD-araB)567, ΔlacZ4787(∷rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514
JW0205-1 dnaQ (mutD) Pol III ε subunit: 3’-> 5’ proofreading F-, Δ(araD-araB)567, ΔdnaQ744∷kan, ΔlacZ4787(∷rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514