Table 1.

Q-PCR forward and reverse primer, as well as MGB probe sequences.

Gene Symbol	Forward Primer (5’-3’)	Reverse Primer (5’-3’)	Probe (5’-3’): 6FAM-Sequence-MGBNFQ MRPS10 only: VIC-Sequence-MGBNFQ
APOA1	ACTCAAAGACAGCGGCAAAGA	GTCCCAGTTGTCCAGGAGCTT	CCTTCAAACTGGGACACATA
APOE	CTGGGTGCAGACACTGTCTGA	GCTGTTCCTCCAGTTCCGATT	TCAACTCCTTCATGGTCTCA
NR1H2	GGGAACTGGACTTTCGTCTCAA	GAAACATTTGCTGGGTGAATGTC	TCTCTAGAATGGTCAACCCAG
NR1H3	TGTGCACGAATGACTGTTCTTTT	CCCTTCTCAGTCTGTTCCACTTC	ATCCGGCCCAGAAAACAAAA
MRPS10	AATGTGCCCAACCTTCATGTC	TCCAGGCAAACTGTTCCTTCA	TGAAGGCCATGCAGTCTCTCAAGTCCC
SQ-PCR forward and reverse primer sequences, and annealing temperature used in PCR reaction.
Gene Symbol	Forward Primer (5’-3’)	Reverse Primer (5’-3’)	Annealing Temperature (°C)
APOA1	CCACGGCCCTTCAGGATGAA	TCAGATGCTCGCTGGCCTTG	64°
APOE	GTCGCTTTTGGGATTACCTG	TGCTCCTTCACCTCGTCCAG	53°
NR1H2	TACAGGACAAGAAGCTACCG	ACATGCTCAATCAGGTTCAG	55°
NR1H3	CAGAGAGGAAGCCAGGATGC	CACTTGCGAAGCCGACACTC	62°
PPIA	CCAGGGTTTATGTGTCAGGG	TGCCTTCTTTCACTTTGCCA	56°