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. 2011 Sep 19;22(2):235–247. doi: 10.1093/glycob/cwr131

Fig. 4.

Fig. 4.

LARGE overexpression in POMT2/DG double-KO cells generated IIH6C4 immunoreactive proteins capable of laminin binding. POMT2/DG double-KO neural stem cells were infected with Ad-LARGE and cultured for 2 days. Glycoproteins were then isolated by WGA-gel and immunoblotted with anti-β-DG and IIH6C4 and assayed for laminin binding by overlay experiment. (A) Wild-type cells showing IIH6C4 immunoreactivity and laminin-binding activity at ∼120 kDa. (B) Overexpressing LARGE in wild-type cells dramatically increased IIH6C4 immunoreactivity and laminin-binding activity at higher-molecular-weight ranges. (C) No IIH6C4 immunoreactivity and laminin binding could be detected in POMT2/DG double-KO cells. (D) Overexpressing LARGE in double-KO cells generated IIH6C4 immunoreactive species capable of laminin-binding activity. Note that β-DG was detected only in wild-type cells but not double-KO cells. Asterisks indicate endogenous laminin that is sometimes detected in the laminin overlay assay.