Table I.
IIH6C4 immunoreactivity of anti-β-DG and VIA4-1 immunoprecipitates upon PNGase F or HFaq treatments
| Cell lines | Untreated | PNGase F | HFaq |
|---|---|---|---|
| β-DG IP | |||
| Wild type | Detected | No change | Undetected |
| Wild type + LARGE | Strong | No change | Undetected |
| POMGnT1 KO | Undetected | Undetected | ND |
| POMGnT1 KO + LARGE | Strong | No change | ND |
| VIA4-1 IP | |||
| Wild type + LARGE | Strong | ND | Significant reduction |
| DG KO + LARGE | Strong | Mild reduction | Significant reduction |
| Double KO + LARGE | Detected | Significant reduction | Significant reduction |
KO, knockout; ND, not done.