Table 1.
Levels of M1 Markers in Bone Marrow-Derived Macrophages
| |
|
|
LPS |
IFNγ+TNFα |
||
|---|---|---|---|---|---|---|
| M1 markers | Young | Aged | Young | Aged | Young | Aged |
| iNOS mRNA (fold change relative to control) | 0.37±0.07 | 0.42±0.21 | 457±106 | 571±137 | 149±12.0 | 155±24.7 |
| IL-6 (pg/mL) | ND | ND | 7,479±937 | 6,916±331 | 4.3±1.7 | 4.2±0.7 |
| IL-1β (pg/mL) | ND | ND | 249±41 | 277±8 | 320±66 | 284±61 |
| TNF-α (pg/mL) | ND | ND | 40,261±5096 | 54,223±4659 | NV | NV |
iNOS mRNA is expressed as fold change over control group, which are unstimulated samples from young mice. Relative mRNA expression was determined via real-time PCR using cDNA from bone marrow-derived macrophages grown from bone-marrow progenitor cells from young and aged mice stimulated for 20 h in the presence of LPS or a combination of IFN-γ and TNF-α. Cytokine values from cell supernatants determined 20 h after stimulation by BioRad multiplex bead array and expressed as pg/mL±standard error of the mean. N=4–5 mice. No significant differences were observed between groups.
iNOS, inducible nitric oxide synthase; interferon-γ, IFN-γ; TNF-α, tumor necrosis factor-α; IL, interleukin; LPS, lipopolysaccharide; PCR, polymerase chain reaction; ND=not detectable, NV=not verified.