Table 2.
Expression of M2 Markers in Bone Marrow-Derived Macrophages
| |
|
|
LPS |
IFNγ+TNFα |
||
|---|---|---|---|---|---|---|
| M2 marker (fold change relative to control) | Young | Aged | Young | Aged | Young | Aged |
| Arg1 | 468±39 | 371±31 | 13.7±3.9 | 19.7±3.9 | 0.2±0.1 | 0.2±0.1 |
| FIZZ1 | 7,204±1,235 | 15,169±2640a | ND | ND | ND | 1.0±0.1 |
| Ym1 | 3±0.3 | 2±0.2 | 0.2±0.09 | 0.2±0.05 | 0.38±0.05 | 0.17±0.03 |
Relative mRNA expression levels are presented as fold change over control group, which are unstimulated samples from young mice. Arg1, FIZZ1, and Ym1 mRNA expression levels were quantified by real-time PCR using cDNA from bone marrow macrophages grown from bone marrow progenitor cells from young and aged mice and were stimulated for 20 h in the presence of IL-4, LPS, and a combination of IFN-γ and TNF-α. N=4–5 mice.
a
p<0.05 compared with young of the same treatment group.
Arg1, arginase-1; FIZZ1, Found In Inflammatory Zone 1.