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. Author manuscript; available in PMC: 2012 Jan 11.
Published in final edited form as: J Immunol. 2010 Oct 20;185(10):5683–5687. doi: 10.4049/jimmunol.1001974

FIGURE 4. Phosphorylation of Vav-1 by αCD244 or by ligation with CD48 expressing targets is reduced in EAT-2A/B−/− NK cells.

FIGURE 4

A. IL-expanded splenic NK cells from wt or EAT-2A/B−/− mice were stimulated with αCD244 mAb for 15 min. Cell lysates were used for immunoprecipitation (IP) with αVav-1 mAb and were probed with anti-p-Tyr mAb (4G10) and reprobed with αVav-1. B, NK cells were stimulated with CD48-expressing P815 cells for 30 min. and analyzed, as in Fig. 4A. C, Lysates of αCD244 activated NK cells form wt or EAT-2A/B−/− mice were analyzed by probing αCD244 immunoprecipitates with anti-p-Tyr mAb (4G10). CD244 was quantitated by reprobing the membrane with αCD244.