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. 2011 Oct 11;20(1):101–108. doi: 10.1038/mt.2011.212

Figure 7.

Figure 7

21-Nucleotide (nt) short-interfering RNA (siRNA)-induced Toll-like receptor-3 (TLR3) signaling activates caspase-3 in retinal pigment epithelium (RPE). (a) Active cleaved caspase-3 was quantified by fluorometric assay in mouse RPE/choroid and hRPE cells after treatment with 21-nt-Luc-siRNA and poly I:C (pIC) compared to phosphate-buffered saline (PBS) control (n = 6 in two independent experiments, fold-upregulation 1.56 ± 0.11 and 2.05 ± 0.39, respectively). (b–d) Immunodetection of active cleaved caspase-3 in albino Balb/C mouse eyes 24 hours after 21-nt-Luc-siRNA or pIC showed increased signal in RPE cells (black arrows) and variable staining in the neural retinal layers (n = 3 in three independent experiments, Bar = 20 µm). *P < 0.05 by Mann–Whitney U-test. Bars represent means ± SEM. siRNA, short-interfering RNA.