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. 2011 Sep 13;20(1):37–45. doi: 10.1038/mt.2011.187

Figure 1.

Figure 1

Virus-encoded angiostatin and tumor vascular endothelial growth factor (VEGF) expression and antiangiogenesis in vivo. (a) U87 flank tumors were harvested 3 days after treatment with phosphate-buffered saline (PBS) (P), G47δ-mAngio (A), or G47δ-empty (E) and analyzed by western blot for: angiostatin [hemagglutinin (HA) epitope], tumor VEGF, and β-actin (loading control). Each lane is a different mouse. (b) Histochemical staining for virus spread (β-galactosidase activity, LacZ-blue) and tumor vascularity (CD31+ brown) in tumors treated with PBS, G47δ-empty, or G47δ-mAngio (hematoxylin nuclear counterstaining). The bar indicates 2 mm. The analysis was done 3 days after treatment and 1 of 3 tumors/group is shown.