Figure 3.

The 3′ U3 deletion of SINΔXbaIGFP is transferred to the 5′ LTR of integrated provirus. (a) Schematic depiction of RT43.2GFP provirus. Arrows indicate primers used to verify the SIN deletion in the 5′ LTR of the provirus, labeled primer A, B and C. Resulting PCR products with expected DNA sizes in base pairs (bp) are given below the schematic. Spaces in between the lines represent PCR product sequences deleted in RT43.2GFP to construct SINΔXbaIGFP. (b) Representative of ethidium bromide stained PCR products separated on an agarose gel from reactions performed with the indicated primer pairs on genomic DNA of MDTF cells exposed to RT43.2GFP or SINΔXbaIGFP. H₂O indicates negative water control for PCR reaction. Center lane is 1 Kb DNA ladder. bp, base pair; LTR, long terminal repeat; MDTF, Mus dunni tail fibroblast; SIN vectors, self-inactivating vectors.