Fig 4.

Laccase inhibition by other sugars also depends on TSP2-1. Sucrose, fructose, mannose, galactose, and maltose, but not lactose, exhibited a repression effect on melanin production in JEC21 (spots on the left in each panel). The disruption of TSP2-1 released the repression (central spots in each panel). The complemented strain was restored to the repression status (spots on the right in each panel). Yeast cells were dropped on Asn salt agar containing NE and 2% of each carbon source at 30°C for 16 or 6 h (for galactose and maltose only, due to fast pigmentation) for melanin production.