Fig 6.

Requirement of TSP2-1 for stress resistance. Yeast cells were serially diluted in 5 μl containing 10⁵, 10⁴, 10³, 10², and 10¹ cells and separately spotted on 2% glucose agar supplement with testing agents at 30°C for 2 days. The mutant strain tsp2-1Δ was highly sensitive to SDS (0.01%) (A) but not to 1% Congo red (B). (C) CFW staining epifluorescence microscopy showed a normal cell wall chitin distribution pattern in the mutant. (D to F) tsp2-1Δ displayed hypersensitivity to osmotic stress from 2.0 M sorbitol, 2.0 M NaCl, or 1.0 M KCl. (G and H) tsp2-1Δ is sensitive to H₂O₂ (1 mM) and NaNO₂ (1 mM). H₂O₂ was added to the medium at a temperature of 55 to 60°C after being autoclaved. (I) Disruption of TSP2-1 resulted in a growth defect at 37°C.