Fig 2.

Thermotolerance of wild type, EVG1064, and EVG1058. (A) Growth of MG1655 and EVG1064 E. coli strains on LB plates at 30°C, 37°C, and 48.5°C; (B) mean generation times of MG1655 and EVG1064 in liquid LB medium plotted as a function of temperature; (C) final cell densities, as measured by OD₆₀₀, at 24 hours for MG1655 and EVG1064 grown in liquid LB medium at various temperatures; (D) resistance to 30-min exposures to elevated temperatures for MG1655 and EVG1064 as measured by growth on LB plates; (E) mean generation times of EVG1058 and EVG1064 in liquid LB medium plotted as a function of temperature. For all panels, error bars indicate ±1 standard deviation. For panels B and E, asterisks denote temperatures at which the growth rates of the two compared strains are significantly different using a 2-tailed, type 2 t test. **, P < 0.005; *, P < 0.05.