Table 1.
Oligonucleotides used to amplify specific regions from the relevant strain's chromosome for the purpose of Sanger sequencing
| Oligonucleotide | Sequence (5′→3′) |
|---|---|
| rpoD_fwd | ATGGAGCAAAACCCGCAGTC |
| rpoD_rev | TTAATCGTCCAGGAAGCTAC |
| ylbE_fwd | ATGTTTACATCAGTGGCGCA |
| ylbE_rev | TCACTTCCCCTGCTCCAGTA |
| kdpD_fwd | ATGAATAACGAACCCTTACG |
| kdpD_rev | TCACATATCCTCATGAAATT |
| ybhN_fwd | ATGAGTAAATCACACCCGCG |
| ybhN_rev | TCACATCGCCGCTTCATTTT |
| rpsA_fwd | ATGACTGAATCTTTTGCTCA |
| rpsA_rev | TTACTCGCCTTTAGCTGCTT |
| pncB_fwd | ATGACACAATTCGCTTCTCC |
| pncB_rev | TTAACTGGCTTTTTTAATAT |
| fabA_fwd | ATGGTAGATAAACGCGAATC |
| fabA_rev | TCAGAAGGCAGACGTATCCT |
| yddB_fwd | ATGAAGCGAGTTCTTATTCC |
| yddB_rev | TTAAAATTTCATGCTGACAT |
| dgsA_fwd | GTGGTTGCTGAAAACCAGCC |
| dgsA_rev | TTAACCCTGCAACAGACGAA |
| pykF_fwd | ATGAAAAAGACCAAAATTGT |
| pykF_rev | TTACAGGACGTGAACAGATG |
| yejM_fwd | ATGGTAACTCATCGTCAGCG |
| yejM_rev | TCAGTTAGCGATAAAACGCT |
| tktB_fwd | ATGTCCCGAAAAGACCTTGC |
| tktB_rev | TCAGGCACCTTTCACTCCCA |
| mreD_fwd | GTGGCGAGCTATCGTAGCCA |
| mreD_rev | TTATTGCACTGCAAACTGCT |
| rpsJ_fwd | ATGCAGAACCAAAGAATCCG |
| rpsJ_rev | TTAACCCAGGCTGATCTGCA |
| perR_fwd | ATGAAGCTCTTAGCAAAAGC |
| perR_rev | TCAACGAATTTTACCCAGAT |
| malQ_fwd | ATGGAAAGCAAACGTCTGGA |
| malQ_rev | CTACTTCTTCTTCGCTGCAG |
| malT_fwd | ATGCTGATTCCGTCAAAACT |
| malT_rev | TTACACGCCGTACCCCATCA |
| yhhZ_fwd | ATGAGTAATATTGTTTACCT |
| yhhZ_rev | TCATTTTGTGTGGTCCATAA |
| malS_fwd | ATGAAACTCGCCGCCTGTTT |
| malS_rev | TTACTGTTGCCCTGCCCAGA |
| spoT_fwd | TTGTATCTGTTTGAAAGCCT |
| spoT_rev | TTAATTTCGGTTTCGGGTGA |
| wzzE_fwd | ATGACACAACCAATGCCTGG |
| wzzE_rev | CTATTTCGAGCAACGGCGGG |
| rffT_fwd | ATGACTGTACTGATTCACGT |
| rffT_rev | TCATGCGACCTCCCTGGCGG |
| glpF_fwd | ATGAGTTAAACATCAACCTT |
| glpF_rev | TTACAGCGAAGCTTTTTGTT |
| treB_fwd | ATGATGAGCAAAATAAACCA |
| treB_rev | TTAAACAATGTCCAGCGTGC |
| idi_fwd | ATGCAAACGGAACACGTCAT |
| idi_rev | TTATTTAAGCTGGGTAAATG |
| yidE_upstream_fwd | CCAATACCTAATCCTATGCC |
| yidE_upstream_rev | TCGTAAACGGTTTACTGCAT |
| ppiC_upstream_fwd | AGCTTGCCGAAATCGGCCCC |
| ppiC upstream_rev | CTTACAGAGGGTATCTTAAT |
| yegTfbaB_upstream_fwd | TCATGTCCGGGGAGATAAAG |
| yegTfbaB_upstream_rev | AAACCGCTTTTACTTAACCA |
| rydC_upstream_fwd | CGCATGATGCCGCGTAAACG |
| rydC_upstream_rev | TGTGAGATCCCCCCTTTCGA |
| yajD_upstream_fwd | TGGCATCTGCGTTGGCTCTG |
| yajD_upstream_rev | AACTCGCGGGAACAGCGACC |
| gltP_upstream_fwd | TATGGCAAAAAGTGATGGAT |
| gltP_upstream_rev | TCGCGGCTGTCGCTATGGTA |
| yqjF_upstream_fwd | ATCCTAATATGCTGGTCCGC |
| yqjF_upstream_rev | GTACCCGCGTAGCCAGTAAT |