Fig 3.
(A) Stepwise improvements of the tyrosine module by replacement of aroK with aroL (Y1) and inserting a second promoter, either PLtetO-1 (Y2) or a combination of the rrnB terminator T1 (large T) and Ptrc (Y3), 5′ of aroA. (B) The SRM results indicate the relative levels of TyrB through AroL as a consequence of the various modifications to the tyrosine module. All cultures were performed under the conditions described in Table 3.
