Table 1.
l-Tyrosine production yields from various E. coli strains engineered within the past 10 years
| Straina | Genotype | Titer (g/liter)b | Ytyr/glu (g/g)b | % Ytc | References |
|---|---|---|---|---|---|
| DPD4193 | K-12; aroH367 tyrR366 tna-2 lacY5 malT384 aroG397(fbr)dtrpE382 Ptrc-tyrA::kanR ΔpheLA | 0.18 (55) | 0.09 | 16 | 33, 35 |
| T2 | K-12; ΔtyrR pCL1920::PLtetO-1-aroGfbr-tyrAfbr-ppsA-tktA | 0.62 (9.7) | 0.124 | 22 | 26 |
| T2-YK | K-12; ΔtyrR pCL1920::PLtetO-1-aroGfbr-tyrAfbr-ppsA-tktA pBR322::PLtetO-1-aroK-ydiB | 0.70 | 0.14 | 25 | 25 |
| PB12CP | JM101; Δ(ptsHI crr) Glc+ pJLB::aroGfbr pTrc::tyrC-pheACM | 0.18 (3) | 0.064 | 12 | 6 |
| ygdT KO | K-12; ΔpheA ΔtyrR ygdT::kan pCL1920::tyrAfbr-aroGfbr | 0.59 | 0.118 | 21 | 38 |
a
KO, knockout.
b
The reported titers and yields (Ytyr/glu, yields of l-tyrosine and l-glucose) were results from the shake flask cultures, except those in parentheses, which were from bioreactors. All cultures grown were grown at 37°C, except PB12CP, which was grown at 30°C.
c
The theoretical yield (% Yt) was calculated based on the maximum value of 0.55 g of l-tyrosine per g of glucose (43).
d
fbr, feedback resistant.