Table 3.

l-Tyrosine production of the various strains constructed in the study^a

Strain	Plasmids	Titer (mg/liter)	mgTyr/gCDWb	% Yt
A	pS0 and pY0	746 ± 18	921 ± 22	27
B	pS0 and pY1	897 ± 28	944 ± 29	33
C	pS4 and pY1	1,150 ± 28	1,307 ± 32	42
D	pS5 and pY1	1,219 ± 24	1,451 ± 29	44
E	pS4 and pY2	908 ± 5	987 ± 5	33
F	pS4 and pY3	2,169 ± 382	2,645 ± 466	79
G	pS5 and pY3	1,310 ± 75	1,617 ± 93	48

^aThe basal strain was E. coli MG1655, which was transformed with the various shikimate and l-tyrosine plasmids (Table 2). The l-tyrosine titer (in mg/liter) reported was the final production obtained within 24 h when glucose had been completely consumed, with the exception of strain F (which was 48 h), in a 50-ml MOPS-M9 minimal medium shake flask culture containing 5 g/liter glucose, shaken at 200 rpm and 37°C. For strain F, the l-tyrosine titer at 24 h was 1,512 ± 27 mg/liter. All cultures were induced with 50 μM IPTG, except for that of strain A. For strain A, the production was maximized at 1 mM IPTG.

^bThe average cell dry weight for all of the strains was 0.38 g/liter per optical-density (OD) unit of culture; it is consistent with the value reported previously (6).