Fig 1.
(a) Relative Vmax of PYK in the presence of 5 mM FBP and varying concentrations of inorganic phosphate, normalized to PYK Vmax in the absence of phosphate. (b) Relative Vmax at pH 7.5 in HEPES buffer, normalized to Vmax in Tris-HCl buffer. (c) Relative activities of LDH (white bars), PYK (gray bars), and GAPDH (black bars) with varying potassium glutamate concentrations normalized to activities in the absence of potassium glutamate. (d) Vmax values with 1/10 the metals present in CDMPC, relative to Vmax values without metals added to the assay buffer. Error bars represent standard deviations of a ratio (see the supplemental material) of average activities from at least three independent dilutions of a single cell extract. ACK, acetate kinase; G6PDH, glucose-6-phosphate dehydrogenase.