Table 2.
Investigation of artificially MTBC-contaminated samples using an enrichment procedure in combination with real-time PCR
| Enrichment time (no. of days) |
CT values (avg of 3 PCR runs) for different contamination levels (CFU/g sample material)a |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Milk (3.8% fat) |
Yoghurt |
Sour cream |
Curd cheese (40% fat) |
|||||||||
| 103 | 102 | 101 | 103 | 102 | 101 | 103 | 102 | 101 | 103 | 102 | 101 | |
| 0 | 36.0 | 39.5 | – | 40.2 | 44.3 | – | 42.4 | 43.5 | – | 40.4 | – | – |
| 7 | 28.9 | 32.7 | 39.9 | 29.7 | 39.3 | – | 32.0 | 36.3 | 40.2 | 31.1 | – | – |
| 14 | 27.3 | 30.6 | 36.7 | 29.3 | 38.5 | 43.2 | 31.5 | 36.0 | 40.0 | 30.7 | – | – |
| 56 | 22.4 | 28.4 | 34.3 | 26.2 | 34.2 | 40.0 | 28.2 | 34.2 | 36.7 | 27.3 | 40.2 | – |
a
–, negative value.