Table 3.

Evaluation of PCR inhibition on the PCR detection of spiked sewage-associated adenoviruses in RHRW, CHTW, and animal fecal samples as opposed to spiked distilled water samples

Sample	CT value (mean ± SD) for PCR
	Undiluted DNA	10-fold dilution	100-fold dilution
Distilled water	25.6 ± 0.4
RHRW
H1	27.1 ± 0.3	26.4 ± 0.3	26.9 ± 0.3
H10	27.2 ± 0.6	26.3 ± 0.4	26.6 ± 0.5
H15	26.7 ± 0.2	26.5 ± 0.5	26.7 ± 0.4
CHTW
H1	26.8 ± 0.1	26.8 ± 0.2	27.9 ± 0.3
H10	28.4 ± 0.3	26.3 ± 0.6	26.0 ± 0.4
H15	26.4 ± 0.4	25.9 ± 0.4	25.8 ± 0.6
Possum feces
P3	26.0 ± 0.3	25.8 ± 0.6	25.8 ± 0.7
P14	26.0 ± 0.6	25.4 ± 0.8	25.9 ± 0.3
P26	28.0 ± 0.1	25.9 ± 0.5	25.9 ± 0.6
Bird feces
B1	26.0 ± 0.4	26.0 ± 0.4	25.9 ± 0.9
B5	26.4 ± 0.5	25.6 ± 0.7	25.4 ± 0.4
B11	25.6 ± 0.2	25.2 ± 0.8	25.2 ± 0.6