Fig 3.

Confirmation of a phage SFP10 receptor by deletion and complementation of rfaL (A) and Tn5 mutation and complementation of rfbG (B) involved in LPS biosynthesis in S. Typhimurium SL1344. The phage sensitivities of wild-type and mutant strains were tested using an adsorption assay with SFP10 phage. Diamonds, wild-type strain (SFP10 sensitive); squares, E. coli MG1655 (SFP10 resistant). (A) Triangles, ΔrfaL deletion mutant; circles, ΔrfaL deletion mutant complemented with the pUHE21-lacI^q::rfaL expression vector. (B) Triangles, ΔrfbG/Tn5 mutant; circles, ΔrfbG/Tn5 mutant complemented with the pUHE21-lacI^q::rfbG expression vector. The error bars indicate the standard deviations in triplicate experiments.