Table 3.
Comparative analysis of ORFs in host specificity-related regions of three similar bacteriophage genomes
ORF | Predicted function | Lengtha | % Identityb | BLASTP best matches | Receptorc | Reference |
---|---|---|---|---|---|---|
Broad-host-range phage SFP10 | ||||||
SFP_0160 | Conserved hypothetical protein | 770 | 71 over 162 aa | Tailspike protein from Salmonella phage Det7 | LPS | 70 |
SFP_0161 | Probable phage tail fiber protein | 926 | 50 over 688 aa | Putative tail fiber from E. coli O157:H7 phage phiV10 | LPS (O157 antigen) | 54 |
48 over 437 aa | Hypothetical tail fiber from E. coli O157:H7 phage JK06 | ND | ||||
SFP_0162 | Phage tailspike protein | 698 | 51 over 709 aa | Tailspike protein from Salmonella phage Det7 | LPS | 70 |
36 over 620 aa | Tailspike protein from Salmonella phage SETP3 | LPS (O12 antigen) | 18 | |||
SFP_0163 | Conserved hypothetical protein partially similar to phage tailspike protein | 1,167 | 83 over 400 aa | Hemolysin-type calcium-binding protein from Salmonella phage Vi01 | Vi capsular antigen | 57 |
SFP_0164 | Hypothetical protein | 396 | 93 over 396 aa | Putative tail fiber protein from Salmonella phage Vi01 | Vi capsular antigen | 57 |
Salmonella phage Vi01 | ||||||
Vi01_170C | Tail spike protein 1 | 596 | 58 over 163 aa | Hypothetical protein from Shigella phage phiSboM-AG3 | ND | 70 |
55 over 76 aa | Tailspike protein from Salmonella phage Det7 | LPS | ||||
Vi01_171C | Vi maturation-adhesion tailspike | 732 | 61 over 293 aa | Predicted tail fiber protein from Salmonella phage Vi06 | Vi capsular antigen | 57 |
63 over 264 aa | Maturation/adhesion protein from Salmonella phage E1 | Vi capsular antigen | 56 | |||
Vi01_172C | Tailspike 3 | 846 | 52 over 695 aa | Head-binding domain of phage tailspike protein from Escherichia fergusonii ATCC 35469 | ND | |
Vi01_173C | Probable tail fiber | 1,057 | 64 over 281 aa | Hypothetical protein from Shigella phage phiSboM-AG3 | ND | |
Vi01_174C | Putative tail fiber | 396 | 65 over 406 aa | Hypothetical protein from Shigella phage phiSboM-AG3 | ND | |
Shigella phage phiSboM-AG3 | ||||||
orf00207 | Conserved hypothetical phage protein | 594 | 47 over 182 aa | Tailspike protein from Salmonella phage Det7 | LPS | 70 |
orf00210 | Tailspike protein | 753 | 66 over 198 aa | Maturation/adhesion protein from Salmonella phage Vi01 | Vi capsular antigen | 57 |
orf00212 | Tailspike protein | 673 | 57 over 96 aa | Conserved phage protein from Enterobacteria phage Eco1230-10 | ND | 62 |
orf00213 | Conserved hypothetical phage protein | 1,019 | 64 over 442 aa | Hemolysin-type calcium-binding protein from Salmonella phage Vi01 | Vi capsular antigen | 57 |
orf00214 | Hypothetical phage protein | 403 | 65 over 406 aa | Putative uncharacterized protein from Salmonella phage Vi01 | Vi capsular antigen | 57 |
Number of amino acids.
Amino acid (aa) sequence identity.
Predicted phage absorption site. ND, not determined.