Table 3.

Fold improvements in total methyl ketone production^a resulting from genetic modifications and the presence of a decane overlay

Methyl ketone source and strain	Fold improvement for overlay
	EGS895b	EGS560c	EGS084d
Overlay
EGS084	700	76	NAe
EGS560	9.0	NA	NA
Pelletf
EGS084	4,600	500	6.6
EGS560	61	6.6	NA
EGS895	4.7	NA	NA

^aRatios of total methyl ketone concentrations at 39 h. Individual and total methyl ketone concentrations in these strains are presented in Table S3 in the supplemental material.

^bStrain EGS895, β-ketoacyl-CoA overproducing, FadM overexpressing (full description in Table 1).

^cStrain EGS560, β-ketoacyl-CoA-overproducing control without FadM (full description in Table 1).

^dStrain EGS084, fatty acid-overproducing control without FadM (full description in Table 1).

^eNA, not applicable.

^fCell pellet extracted after incubation; no decane overlay used.