Fig 1.

Transcriptional regulation of mprF and dlt expression by GraRS and VraFG. (A) Relative transcription level of mprF in parental Mu50 (1), its ΔvraG mutant (2), and its ΔgraR mutant (3) strains. (B) Relative transcription of the dlt in Mu50 (bar 1), ΔvraG (bar 2), and ΔgraR (bar 3) strains. (C) Effect of vraG mutation on graRS transcription. RNA samples from parental Mu50, its ΔvraG mutant, and its complemented ΔvraG mutant containing pEPSA5::vraFG were isolated at 2 h postinoculation (exponential growth phase) and subjected to a qRT-PCR analysis. *, P < 0.01.