Table 2.
Effect of graR and vraG mutations on CAP susceptibilities and PL profiles
| Strain | Mean ± SDa |
|||
|---|---|---|---|---|
| % Survival after 2-h exposure to: |
% L-PG among overall PL content |
|||
| 3 μg of RP-1/ml | 20 μg of hNP-1/ml | Total L-PG (inner + outer) | Outer L-PG only | |
| S. aureus Mu50 (parental) | 100† | 100 | 12.89 ± 0.16 | 4.42 ± 1.13 |
| Mu50 ΔvraG | 65.23 ± 2.03* | 79.47 ± 7.63* | 9.46 ± 2.81* | 1.57 ± 0.4* |
| Mu50 ΔgraR | 33.91 ± 0.81* | 59.78 ± 4.01* | 9.69 ± 0.49* | 1.65 ± 0.24* |
| S. aureus COL (parental) | 100† | 100 | 15.63 ± 0.33 | 5.28 ± 0.05 |
| COL ΔvraG | 49.17 ± 12.96* | 32.19 ± 2.77* | 12.65 ± 1.09* | 4.29 ± 1.83 |
| COL ΔgraR | 53.33 ± 18.86* | 53.23 ± 10.09* | 12.04 ± 1.12* | 4.31 ± 0.86 |
a
*, P < 0.05 versus the parental strains. †, Parental strains were normalized to 100%.