Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Reference |
|---|---|---|
| E. coli K12 strains | ||
| MS427 | MG1655flu | 52 |
| OS56 | MG1655flu attB::bla-gfp GFP+, Ampr | 64 |
| MS427(pBAD) | MS427 pBADMycHisA, Ampr | 76 |
| MS427(pUpaC) | pUpaC in MS427, Ampr | This study |
| MS427(pUpaB) | pUpaB in MS427, Ampr | This study |
| OS56(pBAD) | OS56 pBADMycHisA | 76 |
| OS56(pUpaC) | pUpaC in OS56, Ampr Kanr | This study |
| OS56(pUpaB) | pUpaB in OS56, Ampr Kanr | This study |
| BL21 | F−ompT hsdS(rB− mB−) dcm gal | Stratagene |
| MS2930 | BL21 pUpaBTruncated | This study |
| S17-1λpir | RP4-2Tc::mukan::Tn7 λpir; Pir-dependent replication, Mur Kanr | 65 |
| S17-1λpir_pSC189 | pSC189 in S17-1λpir, Ampr Kanr | 12 |
| E. coli CFT073 strains | ||
| CFT073 | Wild-type UPEC isolate | 44 |
| CFT073ara | CFT073 araDAB::zeo, Zeor | This study |
| CFT073amp | CFT073 attB::amp-cfp, Ampr | 18 |
| RExUpaB | CFT073ara camRExBADupaB, Zeor Camr, arabinose inducible UpaB | This study |
| RExUpaC | CFT073ara specRExBADupaC, Zeor Specr, arabinose inducible UpaC | This study |
| CFT073upaB | CFT073upaB::zeo, Zeor | This study |
| CFT073upaC | CFT073upaC::kan, Kanr | This study |
| CFT073PcLupaB | CFT073 kanPcLupaB, constitutively expressed UpaB, Kanr | This study |
| CFT073PcLupaC | CFT073 kanPcLupaC, constitutively expressed UpaC, Kanr | This study |
| CFT073lacIZ | CFT073lacIZ::cam, Camr | This study |
| CFT073lacIZ upaB::lacZ-zeo | CFT073lacIZ::cam upaB::lacZ-zeo, Camr Zeor | This study |
| CFT073lacIZ upaB::lacZ-zeo c1701::kan | CFT073lacIZ::cam upaB::lacZ-zeo c1701::kan, Camr Zeor Kanr | This study |
| CFT073lacIZ upaB::lacZ-zeo c1701::kan pBR322 | CFT073lacIZ::cam upaB::lacZ-zeo c1701::kan pBR322, Camr Zeor Kanr Ampr | This study |
| CFT073lacIZ upaB::lacZ-zeo c1701::kan pH-NS | CFT073lacIZ::cam upaB::lacZ-zeo c1701::kan pH-NS, Camr Zeor Kanr Ampr | This study |
| CFT073lacIZ upaC::lacZ-zeo | CFT073lacIZ::cam upaC::lacZ-zeo, Camr Zeor | This study |
| CFT073lacIZ upaC::lacZ-zeo c1701::kan | CFT073lacIZ::cam upaC::lacZ-zeo c1701::kan, Camr Zeor Kanr | This study |
| CFT073lacIZ upaC::lacZ-zeo c1701::kan pBR322 | CFT073lacIZ::cam upaC::lacZ-zeo c1701::kan pBR322, Camr Zeor Kanr Ampr | This study |
| CFT073lacIZ upaC::lacZ-zeo c1701::kan pH-NS | CFT073lacIZ::cam upaC::lacZ-zeo c1701::kan p-NS, Camr Zeor Kanr Ampr | This study |
| CFT073lacIZ upaB::lacZ-zeo c0421::kan | CFT073lacIZ::cam upaB::lacZ-zeo c0421::kan, Camr Zeor Kanr | This study |
| CFT073lacIZ upaC::lacZ-zeo c0421::kan | CFT073lacIZ::cam upaC::lacZ-zeo c0421::kan, Camr Zeor Kanr | This study |
| CFT073lacIZ upaB::lacZ-zeo c1699::kan | CFT073lacIZ::cam upaB::lacZ-zeo c1699::kan, Camr Zeor Kanr | This study |
| CFT073lacIZ upaC::lacZ-zeo c1699::kan | CFT073lacIZ::cam upaC::lacZ-zeo c1699::kan, Camr Zeor Kanr | This study |
| CFT073lacIZ upaB::lacZ-zeo c2091::kan | CFT073lacIZ::cam upaB::lacZ-zeo c2091::kan, Camr Zeor Kanr | This study |
| CFT073lacIZ upaC::lacZ-zeo c2091::kan | CFT073lacIZ::cam upaC::lacZ-zeo c2091::kan, Camr Zeor Kanr | This study |
| CFT073lacIZ upaB::lacZ-zeo c2411::kan | CFT073lacIZ::cam upaB::lacZ-zeo c2411::kan, Camr Zeor Kanr | This study |
| CFT073lacIZ upaC::lacZ-zeo c2411::kan | CFT073lacIZ::cam upaC::lacZ-zeo c2411::kan, Camr Zeor Kanr | This study |
| CFT073lacIZ upaB::lacZ-zeo c3218::kan | CFT073lacIZ::cam upaB::lacZ-zeo c3218::kan, Camr Zeor Kanr | This study |
| CFT073lacIZ upaC::lacZ-zeo c3218::kan | CFT073lacIZ::cam upaC::lacZ-zeo c3218::kan, Camr Zeor Kanr | This study |
| CFT073lacIZ upaB::lacZ-zeo c3244::kan | CFT073lacIZ::cam upaB::lacZ-zeo c3244::kan, Camr Zeor Kanr | This study |
| CFT073lacIZ upaC::lacZ-zeo c3244::kan | CFT073lacIZ::cam upaC::lacZ-zeo c3244::kan, Camr Zeor Kanr | This study |
| CFT073lacIZ upaB::lacZ-zeo c3744::kan | CFT073lacIZ::cam upaB::lacZ-zeo c3744::kan, Camr Zeor Kanr | This study |
| CFT073lacIZ upaC::lacZ-zeo c3744::kan | CFT073lacIZ::cam upaC::lacZ-zeo c3744::kan, Camr Zeor Kanr | This study |
| CFT073lacIZ upaB::lacZ-zeo c4864::kan | CFT073lacIZ::cam upaB::lacZ-zeo c4864::kan, Camr Zeor Kanr | This study |
| CFT073lacIZ upaC::lacZ-zeo c4864::kan | CFT073lacIZ::cam upaC::lacZ-zeo c4864::kan, Camr Zeor Kanr | This study |
| CFT073lacIZ upaB::lacZ-zeo c5054::kan | CFT073lacIZ::cam upaB::lacZ-zeo c5054::kan, Camr Zeor Kanr | This study |
| CFT073lacIZ upaC::lacZ-zeo c5054::kan | CFT073lacIZ::cam upaC::lacZ-zeo c5054::kan, Camr Zeor Kanr | This study |
| Plasmids | ||
| pUpaB (pOMS14) | upaB gene (c0426) from CFT073 in pBAD/Myc-HisA-kan, Ampr Kanr | This study |
| pUpaC (pOMS12) | upaC gene (c0478) from CFT073 in pBAD/Myc-HisA-kan, Ampr Kanr | This study |
| pBAD30c1701 H-NS | c1701 (H-NS) from CFT073 in pBAD30, Ampr | 37 |
| pBR322 | Ampr Tetr | 8 |
| pH-NS | pBR322 c1701 (H-NS) from CFT073, Ampr | This study |
| pKOBEG | pSC101 ts (replicates at 30°C), araC, arabinose-inducible λredγβα operon, Camr | 9 |
| pSC189 | oriT, Π-dependent oriR6K, mariner-based transposon TnSC189, Ampr Kanr | 10 |