Table 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Plasmids | ||
| pTOPO-TA | Cloning vector | Invitrogen |
| pTA-BRABR1 | pTOPO-TA::wecA | This study |
| pBR322 | Cloning vector | Promega |
| pRL140 | pBR322::wecA | This study |
| pUC-Gm | pUC::Gm | This study |
| pRL144 | pBR322::wecA::Gm | This study |
| pMAK705ts | Temperature-sensitive vector; Cmr | This study |
| pRL145 | pMAK705ts::wecA::Gm | This study |
| pACYC184 | Complementation vector; Tetr | 55 |
| pGEM-T Easy | Commercial cloning vector; Ampr | Promega |
| pDSM643 | pGEM-T Easy::wecA; Ampr | This study |
| pDSM647 | pACYC184::wecA; Tetr | This study |
| E. coli strains | ||
| Top10 | Cloning strain | Laboratory strain |
| DH5α | Cloning strain | Laboratory strain |
| Top10F′ | Cloning strain; F+ | Laboratory strain |
| PR4131 | E. coli DH5α(pRL140) | This study |
| PR4135 | E. coli DH5α(pRL144) | This study |
| PR4136 | E. coli Top10F′(pRL145) | This study |
| S. enterica strains | ||
| DSM644 | Wild-type S. Typhimurium TML | 23 |
| DSM645 | TML ΔwecA; Gentr | This study |
| DSM649 | TML ΔwecA (pDSM647) Gentr Tetr | This study |
| DSM753 | Wild-type S. Typhimurium SL1344 | 62 |
| DSM754 | SL1344 ΔwecA; Gentr | This study |
| DSM732 | SL1344 ΔwecA (pDSM647) Gentr Tetr | This study |