Fig 7.
Fusion of OMVs from A. actinomycetemcomitans strain D7SS with HeLa cell and HGF lipid raft microdomains. (A and B) Colocalization of rhodamine B-R18-labeled OMVs with FITC-conjugated lipid raft marker CtxB in HeLa cells (A) and HGF (B) after 30 min of incubation in the presence or absence of Filipin III (final concentration, 10 μg/ml). Colocalization of OMVs (red) with CtxB (green) is indicated by small white arrows. Scattergrams of red and green pixels were plotted on graphs to obtain the colocalization coefficient (rp) between OMVs and CtxB. Shown is a representative plot. (C and D) HeLa cells (C) and HGF (D) subjected to control treatment (PBS [buffer] instead of OMVs). The merged images show the labeling with both dyes. Confocal Z-stack projections are included in all images. Magnification, ×1,000. Bars = 10 μm.