Fig 2.

Binding and fH inhibition by chimeric human IgG subclass mouse anti-fHbp MAbs. (A) Binding to fHbp by ELISA. (B) Binding to live N. meningitidis bacterial cells of strain H44/76 by flow cytometry. The MAbs were tested at 4 μg/ml. IgG1, black solid lines; IgG2, gray solid lines; IgG3, black dashed lines (the respective binding lines are superimposed). An irrelevant human IgG1 MAb (100 μg/ml) was used as a negative control (dark gray filled histograms). (C) Inhibition of fH binding by flow cytometry using 20% IgG-depleted human serum as a source of fH (∼90 μg/ml) and 2 μg (JAR 3 or JAR 5) or 50 μg (MAb502) of chimeric antibody/ml. Light gray filled area, fH without MAb; dark gray filled area, bacteria without fH as a negative control. Symbols for IgG1, IgG2, and IgG3 MAbs are as defined for panel B.