Fig 3.
Generation of a major CD8 T cell response to YopE69-77 in response to primary infection with Y. pseudotuberculosis. Groups of C57BL/6 mice were infected with wild-type 32777 (wt) or the yopE catalytic inactive mutant (mE) or left uninfected (UI). At the indicated days postinfection, mice were euthanized, and the percentages of YopE69-77-specific CD8 T cells among total CD8 T cell populations in spleen (A) or MLN (B) were determined by flow cytometry and tetramer staining. Each symbol represents the value obtained from one mouse. The data are pooled from 3 independent experiments with cohorts of 1 to 5 mice. When the values obtained from the spleens of mice infected with the wt were compared to those from mice infected with mE at the day 14, the difference was significant (*, P < 0.05, Mann-Whitney test).