Fig 5.

Comparison of weights and bacterial colonization levels of organs in mice infected with the Y. pseudotuberculosis wt or mE strains. Groups of C57BL/6 mice were left uninfected (UI) or infected i.g. with 5 × 10⁷ CFU of the wt or 5 × 10⁸ CFU of mE. At the indicated days postinfection, the weights of spleen (A) and MLN (B) in grams were determined. Bacterial colonization levels in spleens (C) and MLNs (D) was determined by CFU assay (number of log₁₀ CFU/organ). Each symbol represents the value obtained from one mouse. The data shown are the summary of three independent experiments with cohorts of 1 to 5 mice. (C, D) The dashed line indicates the limit of detection. By Mann-Whitney test, “*” indicates P values of less than 0.05, and “**” indicates P values of less than 0.01 when comparing the values obtained from wt-infected mice to those obtained from mice infected with mE at the same time point.