Fig 6.

Pulmonary inflammation in cytokine knockout mice after eight intranasal exposures to A. fumigatus conidia. (A) After 4 weekly exposures, leukocytes were recovered from the airway, and total leukocytes, CD4⁺ T cells, and eosinophils were enumerated in IFN-γ^−/−, IL-4^−/−, and IL-10^−/− mice. The bar graphs are normalized to compare all the experiments in one graph and depict the mean ± SEM of the ratios generated by comparing the cell numbers for a particular knockout mouse to the wild-type controls included with each experiment (n = 3 experiments/knockout, 3 to 5 mice/experiment). *, P < 0.05. (B) The bar graphs depict the means ± SEM of the ratios generated by normalizing to the wild-type controls included with each experiment for the percentage of CD4⁺ T cells that produced cytokine (n = 3 experiments/knockout, 3 to 5 mice/experiment). *, P < 0.05. (C) The production of cytokines by CD4⁺ T cells isolated from the BAL fluid after four conidial challenges was assessed in wild-type and IL-10^−/− mice by intracellular flow cytometry. The density plots show the percentage of cytokine-producing cells within the gated CD4⁺ T cell population.