Table 1.
Primers used in this study
| Primer | Orientationa | Sequence (5′–3′)b | Description |
|---|---|---|---|
| P1 | F | ACCCGGAGGAAGGGATTCACTTAA | PG0352 5′RACE inner primer |
| P2 | R | GCCCTTTGTAGTCCGGCAAGTC | PG0352 5′RACE outer primer |
| P3 | F | GAATTCCTGGTTAGTTTTTGGTTTGTG | PG0352 promoter conformation |
| P4 | R | GGATCCCATTCGAAAACAATTTTATACCG | PG0352 promoter conformation |
| P5 | F | GCTCTTTCAGCTTGGTATAGG | PG0352 upstream region |
| P6 | R | AGATCTGACATAACGTCGAGTCTTCGC | PG0352 upstream region |
| P7 | F | AGATCTACGATCTCTTCGATGTCCGGC | PG0352 downstream region |
| P8 | R | GACCTACCACGAATATCAACC | PG0352 downstream region |
| P9 | F | AGATCTAGCTTCCGCTATTGCTTT | erm cassette |
| P10 | R | AGATCTTTTATCTACATTCCCTTTAGT | erm cassette |
| P11 | F | CACCGCAAATAATACTCTTTTGGCGA | Confirmation of erm insertion |
| P12 | F | TGCCGTTGCAGAAAAGCC | Confirmation of erm location |
| P13 | R | TTGCCGGACATCGAAGAGATCGTC | Confirmation of erm insertion |
| P14 | F | TTGTCGGTTTCTGTTGGCTC | Confirmation of PG0352 deletion |
| P15 | R | AAAGCTTTGCCTCATCGC | Confirmation of PG0352 deletion |
| P16 | F | AGAGCTGTCGCCTGCCTC | PG0352 RT-PCR |
| P17 | R | CTGTCGGCTCTCCTGCCGTC | PG0352 RT-PCR |
| P18 | F | GTCGACATGGCAAATAATACTCTTTT | Overexpression of PG0352 |
| P19 | R | CTGCAGTTGCCGGACATCGAAGAGAT | Overexpression of PG0352 |
a
F, forward primers; R, reverse primers.
b
The engineered restriction enzyme sites are underlined.